Human Mesenchymal Stem Cells-Derived Exosome Mimetic Vesicles Regulation of the MAPK Pathway and ROS Levels Inhibits Glucocorticoid-Induced Apoptosis in Osteoblasts

Background. Long-term extensive use of glucocorticoids will lead to hormonal necrosis the femoral head, and osteoblasts play an important role in prevention osteonecrosis. However, there is no complete cure for head. Mesenchymal stem cell- (MSCs-) derived exosomes are widely used repair various tissue lesions. Therefore, aim this study was investigate mechanism dexamethasone- (DEX-) induced osteoblast apoptosis therapeutic effect human umbilical cord MSC- (hucMSC-) exosome mimetic vesicles (EMVs) on osteoblast-induced by DEX. Methods. The viability primary MC3T3-E1 cells were determined Cell Counting Kit-8 (CCK-8), FITC-Annexin V/PI staining immunoblot. intracellular levels reactive oxygen species (ROS) after DEX treatment measured 2′, 7′ -dichlorodihydrofluorescein diacetate (DCFH-DA) staining. In study, hucMSC-EMVs N-acetyl-l-cysteine (NAC) as measures. expression B-cell lymphoma 2-associated X, Bcl 2, HO-1, nuclear factor erythroid-derived 2-like 2 MAPK- signaling pathway osteogenic cell treated with Dex analyzed immunoblotting. Results. significantly ROS accumulation. MAPK-signaling activated treatment. intervention downregulated DEX-induced activation addition, can reduce Conclusions. Our confirmed that regulates inhibit apoptosis.